nanoHUB-U Nanobiosensors L5.1: Putting the pieces together – Genome Sequencer I

Table of Contents:
00:09 Lecture 5.1: Putting the pieces together – Genome Sequencer- Part 1
01:35 Outline
02:28 Motivation for Genome Sequencing
05:13 Extraordinary Progress
06:58 Solid-state ICs vs. Biochips
07:57 Outline
07:58 Conventional Sequence-by-Synthesis
13:21 Recall: Theory of pH-sensing
15:01 pH-based Genome Sequencers
18:52 Sequencing by Synthesis
20:27 Modeling Approach
23:13 Model Validation
24:50 How does the pH-based sequencer
27:45 Conclusions

This video is part of the nanoHUB-U course “Principles of Electronic Nanobiosensors”. (

This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.

For more details see

Post time: 06-20-2017